Polysaccharide - degrading thermophiles generated by heterologous gene 3 expression in Geobacillus kaustophilus HTA 426 4 5

Submitted to Applied and Environmental Microbiology 1 2 Polysaccharide-degrading thermophiles generated by heterologous gene 3 expression in Geobacillus kaustophilus HTA426 4 5 Hirokazu Suzuki, Ken-ichi Yoshida, and Toshihisa Ohshima 6 7 Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu 8 University, Fukuoka 812-8581, Japan 9 Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 10 Hyogo 657-8501, Japan 11 Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate 12 School, Kyushu University, Fukuoka 812-8581, Japan 13 14 Present address: Faculty of Engineering, Osaka Institute of Technology, Osaka 535-8585, Japan 15 16 Running title: Overexpression system for G. kaustophilus 17 18 19 20 Correspondence to: Hirokazu Suzuki; Functional Genomics of Extremophiles, Department of 21 Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 22 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Tel.: +81 92 642 7609; Fax.: +81 92 23 642 3053; E-mail: [email protected] 24 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.01506-13 AEM Accepts, published online ahead of print on 21 June 2013